RNAseq Laboratory Workshop

GOAL

This training course includes presentations on technology theory and practical experience with the entire laboratory sample prep workflow. Experienced trainers will lead you through using TapeStationand Miseqequipment. By the end of this training, participants will be able to complete the Illumina library preparation process and set up a sequencing run using Illumina MiseqControl Software.

Pre-course Materials
Presentation Slides

Pre-course Materials

You should have received an email including the agenda and pre-course materials for this workshop.

⬣ Illumina Sequencing Overview

⬣ Introduction to MiSeq and the NextSeq

⬣ Best Practices for TruSeq Stranded mRNA

⬣ Overview of Illumina NGS Library Preparation Workflow Overview of Illumina NGS library preparation workflows include understanding the basics of NGS chemistry, library multiplexing and the difference between the library preparations in all NSG methods such as whole genome sequencing (WGS), targeted sequencing (16s sequencing or exome seq), RNA sequencing, Methylation sequencing and ChIP sequencing, etc.

⬣ Understanding Illumina SBS TechnologySequencing by Synthesis (SBS) technology uses four fluorescently labeled nucleotides to sequence the tens of millions of clusters on the flow cell surface in parallel. During each sequencing cycle, a single labeled dNTP is added to the nucleic acid chain, after dNTP incorporation, the fluorescent dye is identified through laser excitation and imaging, then enzymatically cleaved to allow the next round of incorporation. Base calls are made directly from signal intensity measurements during each cycle.